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The normoxia atmosphere (20% O 2 and 5% CO 2 ) was used for the initial three groups, while the hypoxia condition (5% O 2 , 50% CO 2 , and N 2 supplied) was used for the last two groups

Document weekly dose, date, and injection site to maintain consistency

The samples for TEM characterization were prepared by placing a drop of colloidal solution on carbon-coated copper grid and dried at room temperature

The relative expression level of ACSL4 gene was determined with -actin as an internal reference gene by denaturing at 95 C for 15 s, annealing at 60 C for 30 s and extending at 72 C for 30 s for 40 cycles using the following primer sequences (Invitrogen, USA): ACSL4: forward, 5CCGACCTAAGGGAGTGATGA3

ROS oxidize molecules like LDL, which macrophages absorb, forming foam cells and releasing inflammatory mediators